Plants sense and integrate a variety of signals from the environment through different interacting signal transduction pathwaysthat involve hormones and signaling molecules. Using ALTERNATIVE OXIDASE1a (AOX1a) gene expression as a model system of retrograde or stress signaling between mitochondria and the nucleus.
Dr. Zhang Xinhua, as a visiting scholar at La Trobe University in Australia, 2014- 2015, under the guidance of Prof. Whelan James and postdoctoral fellow, De Clercq Inge, with another postdoctoral researcher, Ivanova Aneta, have identified MYB DOMAIN PROTEIN29 (MYB29) as a negative regulator (regulator of alternative oxidase1a 7 [rao7] mutant) in a genetic screen of Arabidopsis (Arabidopsis thaliana). Results showed that rao7/myb29 mutants have increased levels of AOX1a transcript and protein compared to wild type after induction with antimycin A. A variety of genes previously associated with the mitochondrial stress response also display enhanced transcript abundance, indicating that RAO7/MYB29 negatively regulates mitochondrial stress responses in general. Meta-analysis of hormone responsive marker genes and identification of downstream transcription factor networks revealed that MYB29 functions in the complex interplay of ethylene, jasmonic acid, salicylic acid, and reactive oxygen species signaling by regulating theexpression of various ETHYLENE RESPONSE FACTOR and WRKY transcription factors. Despite an enhanced induction ofmitochondrial stress response genes, rao7/myb29 mutants displayed an increased sensitivity to combined moderate light and drought stress. These results uncover interactions between mitochondrial retrograde signaling and the regulation of glucosinolate biosynthesis, both regulated by RAO7/MYB29. This common regulator can explain why perturbation of the mitochondrial function leads to transcriptomic responses overlapping with responses to biotic stress.
This research is published in Plant Physiology, 173: 1824-1834, March 2017 (IF: 6.28, 2016). Read this article: http://www.plantphysiol.org/content/173/3/1824.full.pdf+html
Zhang Xinhua Research interest: Mechanisms of plant stress response and biosynthesis of plant secondary metabolities
